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KMID : 0616120190500020096
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Korean Journal of Pharmacognosy
2019 Volume.50 No. 2 p.96 ~ p.101
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Antioxidant Activity of Helianthus tuberosus L. Flower in Caenorhabditis elegans
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Kim Jun-Hyeong
Lee Ye-Bin
Han Seon-Yeo
Kim Su-JIn
Hwang In-Hyun
Kim Dae-Keun
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Abstract
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Methanol extract of Helianthus tuberosus L. (Compositae) flower was investigated to research the anti-oxidative activity by using a Caenorhabditis elegans model system. Ethyl acetate soluble fraction of the methanol extract showed the most potent DPPH radical scavenging activity. The ethyl acetate fraction was measured on its activities of superoxide dismutase (SOD), catalase, and oxidative stress tolerance with reactive oxygen species (ROS) level in C. elegans. Furthermore, in order to verify if regulation of stress-response gene is responsible for the increased stress tolerance of C. elegans which treated by the ethyl acetate fraction, we checked SOD-3 expression using a transgenic strain. Consequently, the ethyl acetate fraction of H. tuberosus flower increased the catalase and SOD activities in a dose?dependent manner in C. elegans, reduced ROS accumulation dose-dependently. Besides, the ethyl acetate fraction-treated CF1553 worms showed higher SOD-3::GFP intensity than the control group.
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KEYWORD
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Helianthus tuberosus L., Caenorhabditis elegans, Anti-oxidative activity, Catalase, SOD
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